Abstract − Analytical Sciences, 32(6), 705 (2016).
Effective Separation and Simultaneous Determination of Corynoxeine and Its Metabolites in Rats by High-Performance Liquid Chromatography with Tandem Mass Spectrometry and Application to Pharmacokinetics and In Vivo Distribution in Main Organs
Wei WANG,*,** Shaozhong LUO,* Yaping CHEN,* Bo LI,*** and Masao HATTORI**
*School of Pharmaceutical Sciences and Yunnan Provincial Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Chenggong, Kunming, Yunnan 650500, P. R. China
**Division of Metabolic Engineering, Institute of Natural Medicine, University of Toyama, Sugitani, Toyama 930-0194, Japan
***College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, P. R. China
**Division of Metabolic Engineering, Institute of Natural Medicine, University of Toyama, Sugitani, Toyama 930-0194, Japan
***College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, P. R. China
An effective separation and simultaneous determination of corynoxeine and its metabolites using high-performance liquid chromatography with tandem mass spectrometry was developed and validated. The method was applied to pharmacokinetics and in vivo distribution investigations in rats after oral (0.105 mmol kg−1) and intravenous (0.0105 mmol kg−1) doses of corynoxeine. Its brain uptake index was of 3.08 × 10−11 mol g−1 at 3 h and 3.75 × 10−11 mol g−1 at 74 min after oral and intravenous doses, respectively.
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