Analytical Sciences

Abstract − Analytical Sciences, 32(6), 673 (2016).

Development of a Portable Surface Plasmon Resonance Sensor with Multi-Sensing Points Based on the Linear CCD Sensor
Jie ZHAN,*1 Koji FURUI,*2 Hizuru NAKAJIMA,*3 Noriaki KANEKI,*4 Ryoichi ISHIMATSU,*1 Koji NAKANO,*1 Toshihiko IMATO,*1 and Akihide HEMMI*5
*1 Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi, Fukuoka 819-0395, Japan
*2 Production Technology Department Healthcare Business Unit, Konica Minolta, Inc., 2-2-1 Hirosedai, Sayama, Saitama 350-1328, Japan
*3 Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, Tokyo 192-0397, Japan
*4 College of Design and Manufacturing Technology, Graduate School of Engineering, Muroran Institute of Technology, 27-1 Mizumotocho, Muroran 050-8585, Japan
*5 Mebius Advanced Technology Ltd., 3-31-6-107 Nishiogi-kita, Suginami, Tokyo 167-0042, Japan
A portable-type surface plasmon resonance (SPR) sensor, composed from a new optical system for multi-sensing, has been developed to apply to environment analysis, clinical diagnosis etc., where many samples are desired to be analyzed at high throughput. The optical system of the sensor consists of a light-emitting diode, a pair of cylindrical lenses, a pair of collimator lenses, a correction lens, a prism, a polarizer and a linear CCD sensor with 2048 pixels. Reflected light from a sensor chip of the width of 6 mm at a certain incident angle was detected by ca. 618 pixels of the linear CCD sensor as an SPR sensor signal. An SPR sensor signal at a specified incident angle is controllable for optimization by adjusting the position of the CCD sensor. A sensor chip having a 30-stripe linear pattern (100 μm width/stripe) was prepared. The spatial resolution as well as the performance of the sensor were evaluated by using sucrose solutions. As a result, the acquisition of SPR sensor signals from 30 sensing points was successfully achieved with a spatial resolution of 100 μm (distance between 2 sensing points). A lower detection limit of ca. 3.2 – 5.5 × 10−5 RIU with a standard deviation of ±4.5% was obtained by averaging the signals from 6 – 7 pixels of the CCD sensor per one sensing stripe.