Analytical Sciences

An ATP Aptasensor Based on the Peroxidase-like Activity of Hemin/Graphene Oxide Nanosheets

Ying LIANG,*1 Jing SU,*1 Yong HUANG,*1,*2 Xiaohua LI,*1 Yiwen TAO,*1 Chaofeng LU,*1 Jianmeng ZHU,*1 Zhihao BAI,*1,*2 Jinyu MENG,*3 Xiaoling LU,*1,*4 and Yongxiang ZHAO*1

*1 National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning 530021, P. R. China
*2 School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, P. R. China
*3 Biomedical Polymers Laboratory, Soochow University, Suzhou 215123, P. R. China
*4 Department of Immunology, Guangxi Medical University, Nanning 530021, P. R. China

In the present work, a sensitive electrochemical aptasensor was designed for the detection of adenosine triphosphate (ATP) with hemin/graphene oxide nanosheets (HGNs). Firstly, the ATP aptamer was self-assembled on gold electrode surface, and then HGNs were captured to the modified electrode by π-π stacking. The captured HGNs could catalyze the disproportionation reaction of H₂O₂, and produced a detectable electrochemical signal by chronoamperometry. ATP was competitively bound to aptamer which led to the release of HGNs from the electrode surface after adding ATP. The decrease of the electrochemical signal, which was calculated by the difference of amperometric responses before and after incubation of ATP, provided a quantitative signal for ATP detection. A linear correlation was achieved between the difference of the amperometric responses and the logarithmic concentration of ATP ranging from 0.5 to 100 nM with a detection limit of 0.08 nM. Besides, the aptasensor also exhibited good selectivity toward ATP against other analogs.

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