Abstract − Analytical Sciences, 32(3), 371 (2016).
A Rapid and Automated Device for Purifying Nucleic Acids
Masayuki UEHARA,*,** Masato HANAMURA,* Kiyohito YAMADA,* Akemi YAMAGUCHI,* Toshiro MURAYAMA,* Yuji SAITO,* Kotaro IDEGAMI,* and Takayuki HONDA**,***
*Core Technology Development Center, Corporate Research and Development Div., Seiko Epson Corporation, 281 Fujimi, Fujimi-machi, Suwa, Nagano 399-0293, Japan
**Division of Medical Seeds Promotion, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan
***Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto 390-8621, Japan
**Division of Medical Seeds Promotion, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan
***Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto 390-8621, Japan
We have developed a rapid, automated nucleic acid purification device in a single cartridge containing silica-coated magnetic beads. We succeeded in extracting the matrix protein gene of influenza A virus from pharyngeal swab samples within 3 min. The device will be widely applicable to detect a specific gene from the various samples for clinical diagnosis and genetic research.
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