Analytical Sciences

Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips

Saeed MOHAMMADI,*1 Lori Shayne Alamo BUSA,*1 Masatoshi MAEKI,*2 Reza M. MOHAMADI,*3 Akihiko ISHIDA,*2 Hirofumi TANI,*2 and Manabu TOKESHI*2,*4,*5,*6

*1 Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi 8, Kita, Sapporo 060-8628, Japan
*2 Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita, Sapporo 060-8628, Japan
*3 Department of Pharmaceutical Science, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario M5S 3M2, Canada
*4 ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8603, Japan
*5 Innovative Research Center for Preventive Medical Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan
*6 Institute of Innovative for Future Society, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan

We demonstrated a rapid immunoassay for detection of cat cystatin C (cCys-C) which is an important marker for chronic kidney disease in cats, using immuno-pillar chips. The required amount of reagent solution is 200 times smaller than that for the conventional ELISA in the 96-well microplate (0.5 μL versus 100 μL). In addition, the total assay time in the proposed method is more than 12 times shorter than in the conventional method (20 min versus 240 min). The limit of detection in the new method of 3 ng mL^–1 is comparable to that of the conventional method (1 ng mL^–1) and it is in the clinically relevant range.

J-STAGE: View this article in J-STAGE