Analytical Sciences

Oxime Based Selective Fluorescent Sensor for Arsenate Ion in a Greener Way with Bio-Imaging Application

Malay DOLAI,* Rabiul ALAM,* Atul KATARKAR,** Keya CHAUDHURI,** and Mahammad ALI*

*Department of Chemistry, Jadavpur University, Kolkata 700032, India
**Molecular & Human Genetics Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S. C. Mallick Road, Kolkata 700032, India

A newly designed oxime based probe, 2,4-dihydroxyacetophenone-oxime (DHAO), was found to recognize H₂AsO₄^– selectively with ∼3 fold "turn-on" fluorescence enhancement and LOD of 29 μM, K = (2.10 ± 0.54) × 10⁴ M^–1 in purely aqueous medium. The structures of the DHAO and its adduct with H₂AsO₄^– were optimized by DFT calculations. Intracellular imaging of As(V) in HepG2 cells demonstrate the possibilities of in vitro/in vivo applications for selective monitoring of such species.

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