Analytical Sciences

Combinational Effects of Polymer Viscoelasticity and Immobilized Peptides on Cell Adhesion to Cell-selective Scaffolds

Rio KURIMOTO,*1,*2 Kei KANIE,*3 Koichiro UTO,*2 Shun KAWAI,*3 Mitsuo HARA,*4 Shusaku NAGANO,*5 Yuji NARITA,*6 Hiroyuki HONDA,*7 Masanobu NAITO,*1,*8 Mitsuhiro EBARA,*1,*2,*9 and Ryuji KATO*3

*1 Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
*2 International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
*3 Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, Aichi 464-8601, Japan
*4 Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya, Aichi 464-8603, Japan
*5 Nagoya University Venture Business Laboratory, Nagoya University, Furo-cho, Chikusa, Nagoya, Aichi 464-8603, Japan
*6 Department of Cardiac Surgery, Nagoya University Graduate School of Medicine, 65 Turumai-cho, Showa, Nagoya, Aichi 466-8550, Japan
*7 Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya, Aichi 464-8603, Japan
*8 Structural Materials Unit, Research Center for Strategic Materials, NIMS, 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047, Japan
*9 Graduate School of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo 125-8585, Japan

Immobilization of functional peptides on polymer material is necessary to produce cell-selective scaffolds. However, the expected effects of peptide immobilization differ considerably according to the properties of selected polymers. To understand such combinational effects of peptides and polymers, varieties of scaffolds including a combination of six types of poly(ε-caprolactone-co-D,L-lactide) and four types of cell-selective adhesion peptides were fabricated and compared. On each scaffold, the scaffold properties (i.e. mechanical) and their biological functions (i.e. fibroblast-/endothelial cell-/smooth muscle cell-selective adhesion) were measured and compared. The results showed that the cell adhesion performances of the peptides were considerably enhanced or inhibited by the combination of peptide and polymer properties. In the present study, we illustrated the combinational property effects of peptides and polymers using multi-parametric analyses. We provided an example of determining the best scaffold performance for tissue-engineered medical devices based on quantitative data-driven analyses.

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