Analytical Sciences

Facile Preparation of Photoactivatable Surfaces with Tuned Substrate Adhesiveness

Yoshihisa SHIMIZU,* Masao KAMIMURA,* Shota YAMAMOTO,* Shimaa A. ABDELLATEF,* Kazuo YAMAGUCHI,** and Jun NAKANISHI*

*WPI Research Initiative for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
**Department of Chemistry, Faculty of Science, Research Institute for Photofunctionalized Materials, Kanagawa University, 2946 Tsuchiya, Hiratsuka, Kanagawa 259-1293, Japan

This paper describes a facile method for the preparation of photoactivatable substrates with tuned surface density of an extracellular matrix peptide to resolve the impacts of biochemical and mechanical cues on collective cell migration. The controllability of surface ligand density was validated by cell adhesion and migration tests, complemented with fluorescence observation of an alternative ligand. Depending on the surface ligand density, HeLa cells either kept or lost collective characteristics. The present materials will be useful to address mechanobiology of collective cell migration.

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