Analytical Sciences

Colorimetric Determination of Sulfide in Microsamples

Shogo SUGAHARA,*1 Mai SUZUKI,*1 Hiroshi KAMIYA,*2 Masumi YAMAMURO,*3 Hitoshi SEMURA,*4 Yukiko SENGA,*5 Michiko EGAWA,*1 and Yasushi SEIKE*1

*1 Graduate School of Science and Engineering, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan
*2 Shimane Prefectural Institute of Public Health and Environmental Science, 582-1 Nishihamasada, Matsue, Shimane 690-0122, Japan
*3 Institute of Geology and Geoinformation, Geological Survey of Japan (GSJ), AIST, Central 7, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8567, Japan
*4 Inland Water Fisheries and Coastal Fisheries Division, Shimane Prefectural Fisheries Technology Center, 1659-1 Okinoshima, Sono-cho, Izumo, Shimane 691-0076, Japan
*5 Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan

A method for determination of hydrogen sulfide in microsamples (200 μL) was developed by modifying the methylene blue method. Samples were collected using a micropipette and were combined with sulfide coloring reagent and 5 mL of 0.1 M HCl in test tubes. Absorbance of the solution was measured spectrophotometrically at 667 nm. This modified method did not require any special labware or technique, and can be used in a variety of research fields.

J-STAGE: View this article in J-STAGE