Abstract − Analytical Sciences, 32(1), 35 (2016).
Evaluation of a Portable Microchip Electrophoresis Fluorescence Detection System for the Analysis of Amino Acid Neurotransmitters in Brain Dialysis Samples
Nathan J. OBORNY,*1,*2 Elton E. Melo COSTA,*2,*3 Leena SUNTORNSUK,*2,*4 Fabiane C. ABREU,*3 and Susan M. LUNTE*1,*2,*5
*1 Department of Bioengineering, University of Kansas, Lawrence, KS 66045, USA
*2 Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS 66047, USA
*3 Institute of Chemistry and Biotechnology, Federal University of Alagoas, Alagoas, Brazil
*4 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
*5 Department of Chemistry, University of Kansas, Lawrence, KS 66045, USA
*2 Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS 66047, USA
*3 Institute of Chemistry and Biotechnology, Federal University of Alagoas, Alagoas, Brazil
*4 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
*5 Department of Chemistry, University of Kansas, Lawrence, KS 66045, USA
A portable fluorescence detection system for use with microchip electrophoresis was developed and compared to a benchtop system. Using this system, six neuroactive amines commonly found in brain dialysate (arginine, citrulline, taurine, histamine, glutamate, and aspartate) were derivatized offline with naphthalene-2,3-dicarboxaldehyde/cyanide, separated electrophoretically, and detected by fluorescence. The limits of detection for the analytes of interest were 50 – 250 nM for the benchtop system and 250 nM – 1.3 μM for the portable system, both of which were adequate for most analyte detection in brain microdialysis samples. The portable system was then demonstrated for the detection of the same six amines in a rat brain microdialysis sample.
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