Analytical Sciences

Simulation Analysis of Positional Relationship between Embryoid Bodies and Sensors on an LSI-based Amperometric Device for Electrochemical Imaging of Alkaline Phosphatase Activity

Yusuke KANNO,*1 Kosuke INO,*1 Kumi Y. INOUE,*1 Atsushi SUDA,*2 Ryota KUNIKATA,*2 Masahki MATSUDAIRA,*3 Hitoshi SHIKU,*1 and Tomokazu MATSUE*1,*4

*1 Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba, Sendai 980-8579, Japan
*2 Japan Aviation Electronics Industry, Ltd., 3-1-1 Musashino, Akishima, Tokyo 196-8555, Japan
*3 Micro System Integration Center, Tohoku University, 519-1176 Aramaki-aza Aoba, Aoba, Sendai 980-0845, Japan
*4 WPI-Advanced Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan

In the present study, we monitored the alkaline phosphatase (ALP) activity of embryoid bodies (EBs) of mouse embryonic stem (ES) cells using a large-scale integration (LSI)-based amperometric device with 400 sensors and a pitch of 250 μm. In addition, a simulation analysis was performed to reveal the positional relationship between the EBs and the sensor electrodes toward more precise measurements. The study shows that simulation analysis can be applied for precise electrochemical imaging of three-dimensionally cultured cells by normalization of the current signals.

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