Analytical Sciences

Interference Reduction in Glucose Detection by Redox Potential Tuning: New Glucose Meter Development

Seong Je CHO,* Chul-Ho CHO,* Kwang Bok KIM,* Min-Hyoung LEE,* Jae Hong KIM,* Suho LEE,* Jaegeol CHO,* Suntae JUNG,* Dong-Min KIM,** and Yoon-Bo SHIM**

*DMC R&D center Samsung Electronics, 129, Samsung-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do 443-742, South Korea
**Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan National University, Busan 609-735, South Korea

A new glucose meter was developed employing a novel disposable glucose sensor strip comprising a nicotinamide adenine dinucleotide-glucose dehydrogenase (NAD-GDH) and a mixture of Fe compounds as a mediator. An iron complex, 5-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)-1,10-phenanthroline iron(III) chloride (Fe-PhenTPy), was synthesized as a new mediator for the NAD-GDH system. Due to the high oxidation potential of the mediator, the detection potential was tuned to be more closely fitted toward the enzyme reaction potential, less than 400 mV (vs. Ag/AgCl), by mixing with an additional iron mediator. The impedance spectrometry for the enzyme sensor containing the mixed mediators showed an enhanced charge transfer property. In addition, a new cartridge-type glucose meter was manufactured using effective aligned-electrodes, which showed an enhanced response compared with conventional electrode alignment. The proposed glucose sensor resulted in a wide dynamic range in the concentration range of 30 – 500 mg dL⁻¹ with a reduced interference effect and a good sensitivity of 0.57 μA mM⁻¹.

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