Analytical Sciences

Label-free Detection of Antigen Protein Using a Metal Mesh Device Surface-modified by an Antibody

Hirokazu SETO,* Seiji KAMBA,** Takashi KONDO,** Yuichi OGAWA,*** Yu HOSHINO,* and Yoshiko MIURA*

*Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi, Fukuoka 819-0395, Japan
**Murata Manufacturing Co., 1-10-1 Higashikotari, Nagaokakyo 617-8555, Japan
***Graduate School of Agriculture, Kyoto University, Kitashirakawaoiwake-cho, Sakyo, Kyoto 606-8502, Japan

A biosensor for protein detection was developed using antibody-immobilized metal mesh devices. Antihemoglobin antibodies were covalently immobilized on a metal mesh device. Extraordinary transmission with a dipped structure was observed for a metal mesh device immobilized with antihemoglobin antibodies as well as for the original metal mesh device. Hemoglobin in the mixture solution containing albumin at a hundred-fold concentration was detectable using antihemoglobin-immobilized MMDs. The detectability using the antihemoglobin-immobilized metal mesh device was similar to that of a commercially-available kit for the qualitative determination of hemoglobin.

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