Analytical Sciences

Simple Pretreatment and HILIC Separation for LC-ESI-MS/MS Determination of Adenosine in Human Plasma

Hiroya MURAKAMI,* Erina OTANI,* Tomoko IWATA,* Yukihiro ESAKA,* Takuma AOYAMA,** Masanori KAWASAKI,** Toshiki TANAKA,** Shinya MINATOGUCHI,** and Bunji UNO*

*Gifu Pharmaceutical University, Daigaku-nishi, Gifu 501-1196, Japan
**Department of Cardiology, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan

A simple pretreatment method and separation mode for the LC-ESI-MS/MS determination of adenosine in human plasma have been developed. Deproteinization by acetonitrile and ultrafiltration followed by chromatographic separation with a hydrophilic interaction chromatographic (HILIC) column give a highly sensitive MS/MS response without ionic suppression caused by the matrix compounds in human plasma. In addition, the presence of ammonium acetate in the mobile phase contributes to high sensitivity in MS/MS detection, facilitating the ionization of adenosine. This method seems to be amenable to the treatment of many samples in clinical practice.

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