Analytical Sciences

Construction of an Electrode Modified with Gallium(III) for Voltammetric Detection of Ovalbumin

Kazuharu SUGAWARA,* Makoto OKUSAWA,** Yusaku TAKANO,** and Toshihiko KADOYA*

*Maebashi Institute of Technology, 460-1Kamisadori, Maebashi 371-0816, Japan
**Faculty of Education, Gunma University, 4-2 Aramaki, Maebashi 371-8510, Japan

Electrodes modified with gallium(III) complexes were constructed to detect ovalbumin (OVA). For immobilization of a gallium(III)-nitrilotriacetate (NTA) complex, the electrode was first covered with collagen film. After the amino groups of the film had reacted with isothiocyanobenzyl-NTA, the gallium(III) was then able to combine with the NTA moieties. Another design featured an electrode cast with a gallium(III)-acetylacetonate (AA) complex. The amount of gallium(III) in the NTA complex was equivalent to one-quarter of the gallium(III) that could be utilized from an AA complex. However, the calibration curves of OVA using gallium(III)-NTA and gallium(III)-AA complexes were linear in the ranges of 7.0 × 10⁻¹¹ – 3.0 × 10⁻⁹ M and 5.0 × 10⁻¹⁰ – 8.0 × 10⁻⁹ M, respectively. The gallium(III) on the electrode with NTA complex had high flexibility due to the existence of a spacer between the NTA and the collagen film, and, therefore, the reactivity of the gallium(III) to OVA was superior to that of the gallium(III)-AA complex with no spacer.

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