Analytical Sciences

PNA-tagged Peptide Microarrays for Ratiometric Activity Detection of Cellular Protein Kinases

Hiromu IKEDA,* Yoshihiro YAYAMA,* Akito HATA,* Jumpei KAMIMOTO,* Tatsuhiro YAMAMOTO,*** Takeshi MORI,*,** and Yoshiki KATAYAMA*,**

*Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi, Fukuoka 819-0395, Japan
**Center for Future Chemistry, Kyushu University, 744 Moto-oka, Nishi, Fukuoka 819-0395, Japan
***Institute of System, Information Technologies and Nanotechnologies, 203-1 Moto-oka, Nishi, Fukuoka 819-0385, Japan

Peptide microarrays can be used to measure the activity of multiple protein kinases (PKs), which can be used to elucidate kinomics for drug discovery and diagnosis. Here we demonstrated a new microarray for ratiometric detection of the activity of PKs using peptide nucleic acid (PNA)-tagged peptides labeled with two different fluorophores, Cy3 and Cy5. We successfully detected cellular PK activities based on ratiometry, and applied the system for evaluation of an inhibitory drug.

J-STAGE: View this article in J-STAGE