Analytical Sciences

Quantification of Antioxidants by Using Chlorpromazine Hydrochloride: Application of the Method to Food and Medicinal Plant Samples

Padmarajaiah NAGARAJA, Narayanan ARADHANA, Aandamurthy SUMA, Anantharaman SHIVAKUMAR, and Nelligere Arkeshwaraiah CHAMARAJA

Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore-570006, Karnataka, India

Chlorpromazine hydrochloride (CPH) (3-(2-chloro-phenothiazine-10-yl)-propyl] dimethylamine hydrochloride) has been the subject of a large number of studies employing a broad spectrum of oxidants, and chosen to examine the course of electron transfer reactions. We report on a method to determine the antioxidant activity of some food and medicinal plants using the oxidation of CPH by chromium(VI) to form a stable CPH radical in the 1:1 orthophosphoric acid–ethyl alcohol (OPA-EtOH) medium. The pink color of the control solution was measured at λ_max of 530 nm. Nine standard antioxidants have been studied by this method, along with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The EC₅₀, TEC₅₀, antioxidant efficacy and the stoichiometric values for antioxidants have been evaluated. The radical scavenging activity expressed as EC₅₀ ranged from 9.2 μg/mL in Camellia sinensis to 448.18 μg/mL in Cuminum cyminum. The application of a simple and versatile antioxidant capacity assay for dietary polyphenols and medicinal plant extracts, which are commonly used in Ayurveda opens its relevance in the field of antioxidant analysis.

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