Analytical Sciences

Comparison of Glucose Oxidases from Penicillium Adametzii, Penicillium Funiculosum and Aspergillus Niger in the Design of Amperometric Glucose Biosensors

Arunas RAMANAVICIUS,*1,*2 Jaroslav VORONOVIC,*3,*4 Tatiana SEMASHKO,*5 Raisa MIKHAILOVA,*5 Asta KAUSAITE-MINKSTIMIENE,*3,*4 and Almira RAMANAVICIENE*3,*4

*1 Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225, Vilnius, Lithuania
*2 Laboratory of Bionanotechnology, Semiconductor Physics Institute, State Research Institute Center for Physical and Technological Sciences, A. Gostauto 11, LT-01108, Vilnius, Lithuania
*3 Department of Analytical and Environmental Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania
*4 Division of Immunology, State Research Institute Center for Innovative Medicine, Zygimantu 9, LT-01102, Vilnius, Lithuania
*5 Institute of Microbiology, National Academy of Sciences, Belarus, Kuprevich str., 2, 220141, Minsk, Belarus

The properties of amperometric glucose biosensors based on three different glucose oxidases and various redox mediators were evaluated. Glucose oxidases (GOx) from Penicillium adametzii, Penicillium funiculosum and Aspergillus niger and artificial redox mediators, such as ferrocene, ferrocenecarboxaldehyde, α-methylferrocene methanol and ferrocenecarboxylic acid, were used for modifying the graphite rod electrode and amperometrical reagent-less glucose detection. The obtained results were compared using N-methylphenazonium methyl sulphate in the solution. Taking into account the experimental kinetic parameters and the stability of the tested enzymatic electrodes, GOx from Penicillium funiculosum proved to be more suitable for glucose biosensor design in comparison with other evaluated enzymes.

J-STAGE: View this article in J-STAGE