Analytical Sciences

A Facile Preparation of Glass-supported Lipid Bilayers for Analyzing Molecular Dynamics

Yuma ITO,*,** Kumiko SAKATA-SOGAWA,*,** and Makio TOKUNAGA*,**

*Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, Midori, Yokohama, Kanagawa 226-8501, Japan
**Center for Integrative Medical Sciences, RIKEN, Suehiro-cho, Tsurumi, Yokohama, Kanagawa 230-0045, Japan

Many research programs focus on the molecular dynamics of living cells. This research requires cells to be adhered to a substrate while retaining the innate motility of their surface molecules. Lipid bilayer-based systems fulfill this requirement, although current methods are complicated and their utility is limited. We developed a simple and rapid method for reproducible preparation of homogeneous glass-supported lipid bilayers. Our method provides a facile means for bioimaging and analysis of molecular dynamics in living cells.

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