Analytical Sciences

Development of a Membrane-based Microwave-mediated Electrochemical ELISA Method for TNF-α Detection in Patients with Periodontitis

Irmina DIALA,*1 Shinobu SATO,*1,*2 Michihiko USUI,*3 Keisuke NAKASHIMA,*3 Tatsuji NISHIHARA,*4 and Shigeori TAKENAKA*1,*2

*1 Research Center for Bio-microsensing Technology, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata, Kitakyushu, Fukuoka 804–8550, Japan
*2 Department of Applied Chemistry, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata, Kitakyushu, Fukuoka 804–8550, Japan
*3 Division of Periodontology, Department of Oral Function, Kyushu Dental University, 2-6-1 Manazuru, Kokura-kita, Kitakyushu, Fukuoka 803–8580, Japan
*4 Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, 2-6-1 Manazuru, Kokura-kita, Kitakyushu, Fukuoka 803–8580, Japan

The tumor necrosis factor-α (TNF-α) is implicated in periodontal disease, and there was an attempt to quantitate it by a membrane-based microwave-mediated electrochemical enzyme-linked immunosorbent assay (MMeELISA) using p-aminophenyl phosphate (pAPP) with an over-all time of 1.5 h. A differential pulse voltammetric signal increased linearly with an increase in the amount of TNF-α with a detection limit of 0.48 pg mL⁻¹. This bio-sensing platform revealed a significant difference in the TNF-α level between GCF samples from periodontal patients and healthy volunteers.

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