Analytical Sciences

Two-dimensional Mapping Using Different Chromatographic Separations Coupled with Mass Spectrometry for the Analysis of Ginsenosides in Panax Ginseng Root and Callus

Issey OSAKA,*1 Hirotaka HISATOMI,*2 Yumiko UENO,*2 Shu TAIRA,*3 Yuko SAHASHI,*4 Hideya KAWASAKI,*2 and Ryuichi ARAKAWA*2

*1 Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa, Yokohama, Kanagawa 236-0027, Japan
*2 Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan
*3 Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University, Matsuoka, Yoshida, Fukui 910-1195, Japan
*4 Nitto Denko Corporation, 1-1-2 Shimohozumi, Ibaraki, Osaka 567-8680, Japan

Two-dimensional (2D) mapping using different chromatographic separations coupled with mass spectrometry is a rapid and simple method for the analysis of a mixture using conventional liquid chromatography mass spectrometry. The 2D map could be created from two different chromatograms obtained with the same detector and different columns or separation methods. In this study, 2D mapping was applied to the analysis of components contained in Panax ginseng, and was evaluated in terms of its effectiveness in the separation of these components. The several glycosides included in Panax ginseng could not be sufficiently separated by one-dimensional chromatography with a reverse phase or a hydrophilic interaction chromatography (HILIC) column, but the components of Panax ginseng could be separated and visualized as a component pattern by 2D mapping. We showed that the components contained in the calli and their quantities were altered by the culture conditions in which the calli were grown by 2D mapping. 2D mapping is expected to be a useful method for visualizing complex component patterns found in glycosides and unknown compounds in foods.

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