Analytical Sciences

Diffusion-controlled Detection of Glucose with Microelectrodes in Mediated Bioelectrocatalytic Oxidation

Tatsuo NODA,* Mizue WANIBUCHI,* Yuki KITAZUMI,* Seiya TSUJIMURA,*,** Osamu SHIRAI,* Masahiro YAMAMOTO,*** and Kenji KANO*

*Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan
**Division of Materials Science, Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-5358, Japan
***Department of Chemistry, Konan University, 8-9-1 Okamato, Higashi-Nada, Kobe, Hyogo 658-8501, Japan

This paper describes a diffusion-controlled electrolysis of glucose with mediated bioelectrocatalysis at microdisk-electrodes. Under conditions of an extremely fast enzyme reaction, compared with the diffusion of glucose, the current in potential-step chronoamperometry reaches an almost steady state within 10 s, and is proportional to the glucose concentration. The current can be detected at +0.1 V (vs. Ag|AgCl) with 9,10-phenanthrenequinone as a mediator. The addition of carboxymethylcellulose increased the linear range up to 10 mM.

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