Analytical Sciences

Determination of the Carbon, Hydrogen and Nitrogen Contents of Alanine and Their Uncertainties Using the Certified Reference Material L-Alanine (NMIJ CRM 6011-a)

Nobuyasu ITOH,* Ayako SATO,**,*** Taichi YAMAZAKI,* Masahiko NUMATA,* and Akiko TAKATSU*

*National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Umezono, Tsukuba, Ibaraki 305-8563, Japan
**A-Rabbit-Science Japan Co., Ltd., 5-4-21 Nishihashimoto, Midori, Sagamihara, Kanagawa 252-0131, Japan
***School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka 422-8526, Japan

The carbon, hydrogen, and nitrogen (CHN) contents of alanine and their uncertainties were estimated using a CHN analyzer and the certified reference material (CRM) L-alanine. The CHN contents and their uncertainties, as measured using the single-point calibration method, were 40.36 ± 0.20% for C, 7.86 ± 0.13% for H, and 15.66 ± 0.09% for N; the results obtained using the bracket calibration method were also comparable. The method described in this study is reasonable, convenient, and meets the general requirement of having uncertainties ≤ 0.4%.

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