Analytical Sciences

Development of Electrochemical Microbiochip for the Biological Diagnosis of Neisseria gonorrhoeae

Seung Yong LEE,* Je-Sik JEONG,* Jeong Jin AHN,* Seung Woo LEE,** Hyemyung SEO,*** Yoomin AHN,** and Seung Yong HWANG*

*Department of Bio-Nanotechnology, Graduate School, Hanyang University, Ansan, Gyeonggi-do 426-791, Korea
**Department of Mechanical Engr. Graduate School, Hanyang University, Seoul 133-791, Korea
***Department of Molecular and Life Science, Hanyang University, Ansan, Gyeonggi-do 426-791, Korea

A sexually transmitted disease is an illness that has a high probability of transmission between humans or animals who have sexual contact. Our research is based on the development of a microbiochip for Neisseria gonorrhoeae (N.G.). In our study, we have employed fusion technology between microarray technology and a microfluidic system for quantitative analysis of N.G. A great deal of attention has been focused on electrochemical detection by using a DNA probe, which is a specific DNA sequence and binds to a target biomolecule, because of high affinity, ease of usage, and fast measurement. The microbiochip consisted of two electrode systems and microchannel based PDMS. Our detection principles use electrochemical detection. Consequently, our microbiochip detected 5 ng/mL of N.G. and the correlation rate was over 0.95. We can produce a microbiochip, which could bind to a DNA probe and detect sample of interest. We expect that our electrobiochemical chip will be used for the development of a portable device.

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