Abstract − Analytical Sciences, 28(4), 319 (2012).
Vertical Immobilization of Viable Bacilliform Bacteria into Polypyrrole Films
Shiho TOKONAMI,* Kenta SAIMATSU,** Yuh NAKADOI,* Masakazu FURUTA,*** Hiroshi SHIIGI,** and Tsutomu NAGAOKA**
*Nanoscience and Nanotechnology Research Center, Research Organization for 21st Century, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai 599-8570, Japan
**Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai 599-8570, Japan
***Radiation Research Center, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai 599-8570, Japan
**Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai 599-8570, Japan
***Radiation Research Center, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai 599-8570, Japan
We have successfully developed a novel technique for inserting viable bacilliform bacteria into polypyrrole films. All of the five different bacterial cells (Pseudomonas aeruginosa, Acinetobacter calcoaceticus, Serratia marcescens, Bacillus subtilis and Escherichia coli) studied in this work were inserted normal to the film surface, and the viability of P. aeruginosa was unaffected by this immobilization procedure. It was also found that the polypyrrole layer was important to keep the cells alive.
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