Abstract − Analytical Sciences, 28(11), 1109 (2012).
Control of the Electrostatic Effect on DAM-adsorbent for the Water-soluble Compounds by HPLC
Waka KAMICHATANI,*,** Yoshinori INOUE,* and Atsushi YAMAMOTO**
*Products Planning & Development Department, R&D Center, Nippon Filcon Co., Ltd., 2220 Ohmaru, Inagi, Tokyo 206-8577, Japan
**College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan
**College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan
The effect of the mobile phase pH on the control of the electrostatic interaction was evaluated on a column packed with water-holding adsorbent on which diallylamine-maleic acid copolymers were immobilizing. The adsorbent showed extraordinary retention behaviors of water-soluble solutes under acidic conditions, however, their behavior became stable along with increasing pH. Hydrating water contents tended to level off at pH above 8. Thus, the electrostatic interaction with the stationary phase can be controlled by adjusting the mobile phase pH above 8. In this region, the retention of water-soluble solutes appears to be mainly governed by the hydrophilic partition interaction.
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