Analytical Sciences

Separation of Polyprenyl Phosphate Oligomerhomologues by Reversed-Phase Ion-Pair High-Performance Liquid Chromatography

Vyacheslav V. KOZLOV* and Leonid L. DANILOV**

*Laboratory of Natural Immunity, Department of Immunology, N.F. Gamaleya Research Institute of Epidemiology and Microbiology, 18 Gamalei Str., Moscow 123098, Russia
**Laboratory of Carbohydrate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, Moscow 119991, Russia

A simple and rapid method of reversed-phase ion-pair high-performance liquid chromatography (HPLC) with UV-detection on a common C₁₈ column in an isocratic mode with the addition of tetrabutylammonium phosphate for the separation of polyprenyl phosphate oligomerhomologues has been developed. The method was successfully applied to assay the composition of polyprenyl phosphates obtained from polyprenols isolated from mulberry (Morus alba) leaves and fir (Abies sibirica) needles (the active components of veterinary drugs Gamapren and Phosprenyl correspondingly).

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