Analytical Sciences

Development of a N-Acetyl-β-D-glucosaminidase (NAG) Assay on a Centrifugal Lab-on-a-compact-disc (Lab-CD) Platform

Yoshihide TANAKA,* Seira OKUDA,** Ayumi SAWAI,** and Shigeo SUZUKI**

*Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563–8577, Japan
**Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577–8502, Japan

A centrifugal microfluidic platform, which is also known as lab-on-a-compact-disc (Lab-CD), was developed for use as a urinary N-acetyl-β-D-glucosaminidase (NAG) activity assay. In this work, Lab-CD design, centrifugal operations and analytical procedures were established. Automated liquid handling on Lab-CD processes, such as fluid transport, sample metering, mixing, and fluorescence detection are accomplished using a portable Lab-CD system. The linearity of the NAG assay using 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MU-GlcNAc) was found to be acceptable in the range of 2.5 to 20 U L⁻¹; the relative standard deviations for the fluorescence intensity of eight samples (7.5 U L⁻¹) was 6.4%. Clinical diagnostics is one of the most promising applications for Lab-CD technologies. All the benefits of miniaturization, such as reduced sample requirement, reduced reagent consumption and automation, are realized in this investigation.

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