Analytical Sciences

Simultaneous Determination of Ionic and Neutral Preservatives by Inline Dialysis-ion Chromatography Coupled with a Hydrophobic-ion Exchange Mixed Mode Column^§

Yasuo NAKASHIMA,* Seiichi SUZUKI,* Makiko YAMAZAKI,* Yoshinori INOUE,** Yuta FUKATSU,*** and Atsushi YAMAMOTO***

*Metrohm Japan Ltd., 18-7-2 Ikenohata, Taitoh, Tokyo 110-0008, Japan
**Nippon Filcon Co., Ltd., 2220 Ohmaru, Inagi, Tokyo 208-8577, Japan
***College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan

A quantitative analysis was developed for eight acidic and neutral preservatives in foods and daily necessities using the inline dialysis-IC combined with hydrophobic anion-exchange separation. The eight preservatives were dialyzed by inline dialysis and separated on a hydrophobic anion exchange column. Under the optimized separation conditions, the detection limits (S/N = 3) for the eight preservatives were from 0.08 to 0.66 mg L⁻¹, moreover, a good linearity (R² > 0.998) for each preservative was obtained in the range to 100 mg L⁻¹. Although the dialysis rate of the neutral preservatives was not so high, a good repeatability (RSD, n = 8) of less than 1.5% for the eight preservatives was obtained. The inline dialysis-IC method was applied to the determination of the preservatives in foods and daily necessities. The preservatives were quantified without any interference. The proposed method will be useful for the determination of the preservatives in foods and daily necessities containing high concentration matrices.

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