Analytical Sciences

Rapid and Sensitive Determination of Benzaldehyde Arising from Benzyl Alcohol Used as Preservative in an Injectable Formulation Solution Using Dispersive Liquid–Liquid Microextraction Followed by Gas Chromatography

Hossein Ali MASHAYEKHI,* Mohammad REZAEE,** Shirin Sadeghi GARMAROUDI,*** Naser MONTAZERI,* and Seyed Javad AHMADI**

*Department of Chemistry, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
**Nuclear Fuel Cycle Research School, Nuclear Science & Technology Research Institute, Atomic Energy Organization of Iran, P. O. Box 14395-836, Tehran, Iran
***Department of Chemistry, Science and Research Branch, Islamic Azad University, P. O. Box 1415-775, Tehran, Iran

A rapid and sensitive method has been developed for the determination of benzaldehyde, a toxic oxidation product of the widely used preservative and co-solvent benzyl alcohol in injectable formulations of non-steroidal anti-inflammatory drugs, diclofenac, vitamin B-complex and Voltaren injection solutions by using dispersive liquid–liquid microextraction followed by gas chromatography. This method involves the use of an appropriate mixture of extraction solvent (43.0 μL 1,2-dichloroethane) and disperser solvent (1.0 mL acetonitrile) for the formation of a cloudy solution in a 5.0-mL aqueous sample containing benzaldehyde. The linear range was 1.0 – 1000 μg L⁻¹, and the limit of detection was 0.2 μg L⁻¹ for benzaldehyde.

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