Analytical Sciences

Tube Radial Distribution Phenomenon of Ternary Mixed Solvents in a Microspace under Laminar Flow Conditions

Mari MURAKAMI, Naoya JINNO, Masahiko HASHIMOTO, and Kazuhiko TSUKAGOSHI

Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan

When ternary mixed solutions of water–hydrophilic/hydrophobic organic solvents are fed into a microspace under laminar flow conditions, the solvent molecules are radially distributed in the microspace. The specific fluidic behavior of the solvents is termed “tube radial distribution phenomenon” (TRDP). In this study, water–acetonitrile–ethyl acetate mixed solutions (3:8:4 volume ratio) containing fluorescent dyes, perylene (0.1 mM) and Eosin Y (1 mM), were fed into fused-silica capillary tubes (75 μm i.d.) for investigating the TRDP with a fluorescence microscope-CCD camera under various analytical conditions. The pressure at the observation point for the fluorescence on the tube was changed by altering the flow rates, capillary total lengths, and capillary effective lengths. The obtained fluorescence images showed that the TRDP in the capillary tube created the inner and outer phases, i.e., the organic solvent-rich major inner and the water-rich minor outer phases in this case, providing the kinetic liquid–liquid interface. The formation of the inner and outer phases in the capillary tube was observed at flow rates of 0.5 – 2.0 μL min⁻¹ under the present analytical conditions. We also discuss the specific formation locations of the major and minor solvents.

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