Abstract − Analytical Sciences, 27(5), 461 (2011).
Accurate and Highly Sensitive Screening of DNA-binding Molecules with Primer-dimer Templates Using a Real-time PCR Analyzer
Akira INOUE, Tsunehiro KUMEDA, and Hiroshi NAKAMURA
Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
We have developed a strategy to detect the DNA-binding affinities of chemical compounds with a real-time PCR analyzer. In this system, the DNA-binding affinities of chemical compounds are efficiency detected by PCR with high sensitivity. To obtain accurate information about the DNA-binding affinities of chemical compounds, primer-dimers prepared in advance are used as templates. The denature temperature is settled to around the melting temperature of the templates.
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