Analytical Sciences

A Glucose Sensor Fabricated by Piezoelectric Inkjet Printing of Conducting Polymers and Bienzymes

Yeon Hee YUN,*1 Byung Kook LEE,*1 Ji Suk CHOI,*1 Sungwon KIM,*2 Bongyoung YOO,*3 Yong Shin KIM,*4 Kinam PARK,*2 and Yong Woo CHO*1

*1 Department of Chemical Engineering and Department of Bionanotechnology, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea
*2 Departments of Pharmaceutics and Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
*3 Department of Materials Engineering, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea
*4 Department of Applied Chemistry, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea

Piezoelectric inkjet printing of polymers and proteins holds great promise for fabrication of miniaturized bioelectronic devices, such as biochips and biosensors. In this study, a bienzymatic glucose biosensor prototype based on poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid) (PEDOT-PSS), glucose oxidase (GOD), and horseradish peroxidase (HRP) was fabricated by a piezoelectric inkjet printer. An aqueous bioelectrical ink containing PEDOT-PSS, GOD, and HRP was prepared and printed on an indium-tin-oxide (ITO)-coated poly(ethylene terephthalate) (PET) film. The PEDOT-PSS/GOD/HRP sensor was covered with a cellulose acetate membrane. The use of bienzymatic sensing combined with conducting polymers via piezoelectric inkjet printing showed a synergistic effect resulting in significant amplification of the response signal. The glucose sensor reached steady-state current density within 3 s, indicating a fast response time, and exhibited a linear dose-dependent electrochemical response with high sensitivity. The overall result demonstrates that a glucose sensor with high sensitivity could be readily fabricated by a piezoelectric inkjet printing system.

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