Analytical Sciences

Selective Removal of Endotoxin from a DNA Solution by Cross-linked Cyclodextrin Beads

Masayo SAKATA,* Kana YOSHIMURA,* Itsumi SAKAMOTO,* Masami TODOKORO,** and Masashi KUNITAKE*

*Department of Applied Chemistry & Biochemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan
**Chisso Co. Ltd., 2-2-1 Otemachi, Chiyoda, Tokyo 100-8105, Japan

The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common cationic LPS adsorbents. The γ-CyD polymer beads selectively removed LPS from a DNA solution (50 μg mL⁻¹, pH 6, ionic strength μ = 0.2) containing natural LPS (15 EU mL⁻¹), without the adsorption of DNA. The adsorptions of LPS and DNA were 85% and <1%, respectively.

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