Analytical Sciences

Development and Validation of Spectrophotometric Methods for Estimating Amisulpride in Pharmaceutical Preparations

Sangita SHARMA, Madhurjya NEOG, Vipul PRAJAPATI, Hiren PATEL, and Dipti DABHI

Department of Chemistry, Hemchandracharya North Gujarat University, Patan-384265, Gujarat, India

Five simple, sensitive, accurate and rapid visible spectrophotometric methods (A, B, C, D and E) have been developed for estimating Amisulpride in pharmaceutical preparations. These are based on the diazotization of Amisulpride with sodium nitrite and hydrochloric acid, followed by coupling with N-(1-naphthyl)ethylenediamine dihydrochloride (Method A), diphenylamine (Method B), β-naphthol in an alkaline medium (Method C), resorcinol in an alkaline medium (Method D) and chromotropic acid in an alkaline medium (Method E) to form a colored chromogen. The absorption maxima, λ_max, are at 523 nm for Method A, 382 and 490 nm for Method B, 527 nm for Method C, 521 nm for Method D and 486 nm for Method E. Beer’s law was obeyed in the concentration range of 2.5 – 12.5 μg mL⁻¹ in Method A, 5 – 25 and 10 – 50 μg mL⁻¹ in Method B, 4 – 20 μg mL⁻¹ in Method C, 2.5 – 12.5 μg mL⁻¹ in Method D and 5 – 15 μg mL⁻¹ in Method E. The results obtained for the proposed methods are in good agreement with labeled amounts, when marketed pharmaceutical preparations were analyzed.

J-STAGE: View this article in J-STAGE