Analytical Sciences

Determination of Cyanuric Acid in Milk Powder by Anion-exchange Chromatography

Hui WANG,* Shengjie HOU,* Mingyu DING,* and Jinghua ZHANG**

*Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China
**Beijing Center for Physical and Chemical Analysis, Beijing 100089, P. R. China

An anion-exchange chromatographic method has been developed for simultaneous analysis of cyanuric acid (CA) and five inorganic anions (F⁻, Cl⁻, NO₃⁻, HPO₄²⁻ and SO₄²⁻) in milk powder. The separation was achieved within 15 min on an anion-exchange column with simple elution of Na₂CO₃/NaHCO₃ buffer as mobile phase. Furthermore, the effect of the total concentration of Na₂CO₃/NaHCO₃ buffer on the retention of five inorganic anions was more obvious than that on CA retention, which indicated that CA retention on anion-exchange column depends not only on anion-exchange interaction but also on hydrophobic interactions between CA and anion-exchange column. The linear range of the calibration curve for CA was 0.1 – 100 mg L⁻¹. The detection limit calculated at S/N = 3 was 0.083 mg L⁻¹. The method was successfully applied to the analysis of CA in milk powder.

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