Analytical Sciences

Simultaneous Separation and Determination of Eleven Nucleosides and Bases in Beer, Herring Sperm DNA and RNA Soft Capsule by High-Performance Liquid Chromatography

Shengjie HOU and Mingyu DING

Key Lab of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China

A simple and rapid high-performance liquid chromatography method was developed for the determination of eleven nucleosides and bases in beer, herring sperm DNA and RNA soft capsules. The separation was carried out on an Agilent extend-C₁₈ column with a simple gradient elution of acetonitrile and water as the mobile phase. Good linear relationships between the peak areas and the concentrations of the analytes were obtained. The detection limits for eleven analytes were in the range of 0.007 – 0.037 mg/L by UV detection at 260 nm. The relative standard deviations (RSDs) of the retention times were in the range of 0.78 – 1.85% for intra-day and 0.87 – 1.94% for inter-day, respectively. The RSDs of the peak areas were in the range of 2.71 – 3.22% for intra-day and 3.03 – 3.39% for inter-day, respectively. This method has been successfully applied to simultaneous determination of eleven nucleosides and bases in beer, herring sperm DNA and RNA soft capsules with the recoveries in the range of 93.7 – 108.3%.

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