Analytical Sciences

Chiral Separation of Sertraline with Microemulsion Electrokinetic Chromatography on a Polymer/β-cyclodextrin Assembling Molecular Film Modified Capillary

Hongying CHENG,*,** Baoku HE,** Qianli ZHANG,*,** and Yifeng TU*

*Institute of Analytical Chemistry, Department of Chemistry, Dushu Lake Campus, Suzhou University, Suzhou 215123, P. R. China
**Department of Chemistry, Suzhou University of Science and Technology, Suzhou 215009, P. R. China

A capillary modified by assembling a molecular film was presented for the chiral separation of sertraline by microemulsion electrokinetic chromatography. The assembling molecular film was constructed with poly(diallyldimethylammonium-chloride) and β-cyclodextrin via inclusion complexation. The separation efficiency of cis-trans isomers and enantiomers of sertraline was improved with a running microemulsion that contained the acetonitrile, sodium dodecyl sulfate, n-butanol and n-hexane buffered with sodium tetraborate. The baseline separation of four sertraline cis-trans isomers and enantiomers was achieved under the optimum conditions. The detection limit for isomers and enantiomers of sertraline (1S,4S, 1R,4R, 1S,4R, 1R,4S) was 0.15, 0.15, 0.30, 0.30 mg/L, respectively. The mechanism of chiral separation was studied and it could be applied for the determination of commercial Zoloft tablet samples satisfactorily.

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