Analytical Sciences

Time-dependent Changes in Concentration of Two Clinically Used Acetylcholinesterase Reactivators (HI-6 and Obidoxime) in Rat Plasma Determined by HPLC Techniques after in vivo Administration

Jana ZDAROVA KARASOVA,*1 Ladislav NOVOTNY,*2 Karel ANTOS,*3 Helena ZIVNA,*4 and Kamil KUCA*2,*5

*1 Department of Toxicology, Faculty of Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
*2 Center of Advances Studies, Faculty of Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
*3 Department of Public Health, Faculty of Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
*4 Radioisotope Laboratories and Vivarium, Faculty of Medicine, Charles University, Simkova 870, 500 38 Hradec Kralove, Czech Republic
*5 Department of Chemistry, Faculty of Sciences, J. E. Purkinje University, Horeni 13, 400 96 Usti nad Labem, Czech Republic

A simple and reliable HPLC method for determination of rat plasma levels of clinically used acetylcholinesterase (AChE) reactivators (HI-6 and obidoxime) is presented in our study. Separation was carried out by HPLC using an octadecyl silica stationary phase and a mobile phase consisting of 24% acetonitrile and containing 5 mM sodium octanesulfonate and 5 mM tetramethylammonium chloride (pH 2.3). Following intramuscular administration of equimolar doses of both oximes (22.23 mg/kg), the maximum of HI-6 concentration in rat plasma was reached in about 20 min giving 15.26 ± 1.71 μg/mL. The distribution of obidoxime was fast; the single maximum 23.62 ± 3.563 μg/mL was recorded at about 10 min. HPLC with UV detection presented in our study is a general method which could be applied for quick measurements of bisquaternary AChE reactivators in rat plasma.

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