Analytical Sciences

Measurement of the Transport Activities of Bile Salt Export Pump Using LC-MS

Kana YAMAGUCHI,* Tsuyoshi MURAI,* Hikaru YABUUCHI,** and Takao KUROSAWA*

*Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan
**GenoMembrane, Inc., 75-1 Ono, Tsurumi, Yokohama 230-0046, Japan

The high performance liquid chromatography-electrospray ionization-mass spectrometry method has been applied to the measurement of bile acid transport activities in membrane vesicles obtained from a human bile salt export pump expressing Sf9 cells. The amounts of bile acids transported using the human bile salt export pump expressing Sf9 cells were determined using liquid chromatography-electrospray ionization-mass spectrometry method and the values of the kinetic parameters were determined to be comparable with those obtained using radioisotope-labeled substrates. The developed method was highly useful for the measurements of bile acid transport activities.

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