Analytical Sciences

Practical Method for the Detection of Tetracyclines in Honey by HPLC and Derivative UV-Vis Spectra

Tadao HAKUTA,* Hideyuki SHINZAWA,** and Yukihiro OZAKI***

*Ibaraki Prefectural Institute of Public Health, Ibaraki 310-0852, Japan
**Research Institute of Instrumentation Frontier, Advanced Industrial Science and Technology (AIST), Aichi 463-8560, Japan
***Department of Chemistry, School of Science and Technology, Kwansei-Gakuin University, Hyogo 669-1337, Japan

A technique to detect residual tetracyclines (TCs) in honey by HPLC and derivative UV-Vis spectra is examined in this study. Chromatograms of honey products often experience overlapped peaks and sloping background due to similar retention properties of components or to the presensce of impurities. The derivatives provide resolution of overlapped peaks in an UV-Vis spectrum and eventually bring chromatograms that show pure elution behavior of TCs. Calibration models for oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) are built out of the derivative spectra of standard solutions of honey and their prediction abilities are examined. The results show that the calibration models for OTC, TC and CTC can yield sufficient levels for the determination. The technique reported here may be effectively used for quantitative determination of TCs.

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