Analytical Sciences

Isomeric Oligosaccharides Analyses Using Negative-ion Electrospray Ionization Ion Mobility Spectrometry Combined with Collision-induced Dissociation MS/MS

Tohru YAMAGAKI* and Akihiro SATO**

*SUNTORY Institute for Bioorganic Research, 1-1-1 Wakayamadai, Shimamoto, Mishima, Osaka 618-8503, Japan
**JASCO International Corporation Limited, 1-11-10 Myojin, Hachioji, Tokyo 192-0046, Japan

Ion mobility-mass spectrometry (IM-MS) was applied to analyses of isomeric oligosaccharides linked by α1-4, α1-6, β1-3, and β1-4 glycosyl linkages. Negative-ion electrospray ionization (ESI) quadrupole IM time-of-flight mass spectrometry allowed the combination of IM separation and collision-induced dissociation (CID) MS/MS product ion analysis. Multimer formations of hexa-saccharide linkage isomers differ from each other, and their molecular shapes were analyzed by ion mobility spectrometry (IMS). The product ion spectra of the oligosaccharide isomers were measured by negative-ion CID-MS/MS for each IM separated peak. The spectrum for each isomer was distinct, and their corresponding linkage structures were identified by MS/MS analysis.

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