Analytical Sciences

Enzyme-Release Capillary as a Facile Enzymatic Biosensing Part for a Capillary-Assembled Microchip

Terence G. Henares,* Eiji Maekawa,** Fumito Okubo,* Fumio Mizutani,** Toshio Yao,* Ryuichi Sekizawa,*** and Hideaki Hisamoto*

*Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen, Naka, Sakai, Osaka 599-8531, Japan
**Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako, Hyogo 678-1297, Japan
***Metaboscreen Co. Ltd., 34-1-412 Terakubo, Naka, Yokohama 231-0855, Japan

A simple capillary enzymatic biosensor was developed. This was prepared by simply coating a dissolvable membrane containing enzyme/s on the inner wall of a square glass capillary. An easy measurement was carried out by capillary force sample introduction with concurrent enzyme release and a reaction with a certain substrate. Enzyme-release capillary (ERC) biosensors showed long-term storage stability of at least two weeks for a β-galactoside derivative and glucose. Moreover, this could be integrated on a capillary-assembled microchip (CAs-CHIP) to broaden its multiple analyte sensing potential for clinical diagnostic applications.

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