Analytical Sciences

Determination of Myriocin in Natural and Cultured Cordyceps cicadae Using 9-Fluorenylmethyl Chloroformate Derivatization and High-Performance Liquid Chromatography with UV-Detection

Jiawen YU,* Hongjuan XU,* Zhihong MO,* Huali ZHU,** and Xianbing MAO**

*College of Bioengineering, Chongqing University, Chongqing 400044, China **Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China

A simple and sensitive reversed-phase liquid chromatographic method, based on the precolumn derivatization with 9-fluorenylmethyl chloroformate, was developed for the determination of myriocin. The derivatization reaction was performed in organic solvents of pyridine and tetrahydrofuran at 40°C. Several factors influencing the derivative yield were investigated and optimized. The formed derivative was stable for more than 24 h at room temperature. The detection wavelength was 262 nm. The system offered the following analytical parameters: the limit of detection was 0.045 μg ml⁻¹, the linear correlation coefficient was 0.9963 and the linear range response was from 2.0 to 500.0 μg ml⁻¹. The precision of the method was <2.0%. As a preliminary application, the method has been successfully applied to the determination of myriocin in natural and cultured Cordyceps cicadae.

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