Abstract − Analytical Sciences, 25(6), 829 (2009).
Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Postcolumn Excimer Fluorescence Derivatization
Hideyuki YOSHIDA,*,** Maki SUDO,* Kenichiro TODOROKI,* Hitoshi NOHTA,* and Masatoshi YAMAGUCHI*,**
*Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Johnan, Fukuoka 814-0180, Japan
**Physical Activity Institute, Fukuoka University, 8-19-1 Nanakuma, Johnan, Fukuoka 814-0180, Japan
**Physical Activity Institute, Fukuoka University, 8-19-1 Nanakuma, Johnan, Fukuoka 814-0180, Japan
An LC postcolumn derivatization method for determination of polythiols has been developed. This method involves separation using reversed-phase LC, postcolumn derivatization with N-(1-pyrenyl)maleimide, and excimer fluorescence detection. Analytes with a polythiol structure were converted into corresponding polypyrene-labeled derivatives, and the derivatives exhibited intramolecular excimer fluorescence (440 − 520 nm). In this study, dimercaprol and dithiothreitol were used as model polythiols. This polythiol analysis method is simple; it is also highly selective and sensitive and yields good calibration curves.
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