Abstract − Analytical Sciences, 25(6), 825 (2009).
Activity Measurement of Chitosanase by an Amperometric Biosensor
Hirosuke TATSUMI,* Hajime KATANO,** Tokuji IKEDA,** and Hisashi KIMOTO**
*Department of Bioscience, Fukui Prefectural University, 4-1-1 Matsuokakenjojima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan
**An amperometric biosensor for the detection of glucosamine (GlcN) and chitosan oligosaccharides ((GlcN)n) was introduced for an activity measurement of chitosanase. By using the biosensor, an increase in the anodic current due to the production of GlcN and (GlcN)n by chitosanase was measured in a chitosan solution. The maximum value of the slope of the current increase was proportional to the enzyme concentration up to 1.4 μg mL−1. The present method had the advantages of being simple and rapid over the conventional Elson-Morgan method.
**An amperometric biosensor for the detection of glucosamine (GlcN) and chitosan oligosaccharides ((GlcN)n) was introduced for an activity measurement of chitosanase. By using the biosensor, an increase in the anodic current due to the production of GlcN and (GlcN)n by chitosanase was measured in a chitosan solution. The maximum value of the slope of the current increase was proportional to the enzyme concentration up to 1.4 μg mL−1. The present method had the advantages of being simple and rapid over the conventional Elson-Morgan method.
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