Abstract − Analytical Sciences, 25(6), 765 (2009).
Analysis of β-Sitosterol in Seeds and Nuts Using Pressurized Liquid Extraction and Liquid Chromatography
M. M. DELGADO-ZAMARREÑO, M. BUSTAMANTE-RANGEL, D. MARTÍNEZ-PELARDA, and R. CARABIAS-MARTÍNEZ
Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Salamanca, 37008 Salamanca, Spain
Pressurized liquid extraction (PLE) has been used for the extraction of β-sitosterol from food samples. In the present work, extractions were performed by mixing ground samples with Hydromatrix celite with methanol at 50°C and 110 bar, using two 5-min cycles. After extraction, β-sitosterol was analyzed directly by liquid chromatography with diode array detection. The extraction recovery was 92%, with relative standard deviations of 3.7% for intra-day precision and 4.3% for inter-day precision. The presence of β-sitosterol was confirmed by gas chromatography-mass spectrometry. The proposed method was validated and applied successfully to the determination of free β-sitosterol in seed and nut samples (almonds, pistachios, peanuts, and sunflower seeds).
J-STAGE:
View this article in J-STAGE
Back to the Table of Contents
