Abstract − Analytical Sciences, 25(5), 731 (2009).
Determination of Protein-Ligand Interactions Using Accelerator Mass Spectrometry: Modified Crosslinking Assay
Sang Soo HAH
Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Korea
A highly sensitive detection method for the determination of protein-ligand interactions has been developed. Radiocarbon-labeled 17β-estradiol was incubated with estrogen receptor-alpha; as a selective binding partner, and covalently attached using crosslinking agents, to form covalently linked protein-ligand complexes. After separation using a denaturing gel, the 14C content in the sliced gels was identified by accelerator mass spectrometry. The obtained data demonstrated specific binding of the small molecule to its binding partner. In theory, this method can be applied to most protein-ligand interaction studies.
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