Abstract − Analytical Sciences, 25(5), 727 (2009).
Positive Charged Polymer as a Probe for DNA Determination by Resonance Light Scattering
Juan DU, Huzhi ZHENG, Chengzhi HUANG, Congying WEN, Jianyu HAO, Yufei HU, Dongbo ZHOU, and Li LIU
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, P. R. China
Electrostatic interactions of calf thymus DNA (ct-DNA) with a positive charged polymer were investigated based on measurements of enhanced resonance light scattering (RLS) signals, and were proved by scanning electron microscopy (SEM). The optimal pH value was 7.0 in a phosphate-buffered saline solution (PBS) and the maximum RLS peak located at 344.8 nm. The RLS signal enhancement (ΔIRLS) could be linearly correlated with the concentration of ct-DNA in the range of 0.0900 to 2.70 µg mL-1, and the detection limit (S/N = 3) was 17.0 ng mL-1. The binding ratio and the binding constant between DNA and polymer were also provided.
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