Analytical Sciences

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Aiping FAN,* Zhijuan CAO,* Huan LI,* Masaaki KAI,** and Jianzhong LU*

*School of Pharmacy, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China
**Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan

Chemiluminescent (CL) detection techniques for DNA assays and immunoassays have become very popular in recent years. This review discusses recent advances in those CL assays that have occurred over the last few years. In the monoplex assay section, different classes of CL labels including nanoparticle, DNAzyme, acridinium ester, enzyme and luminol-based CL assays are reviewed concerning the detection of DNAs and proteins. In the multiplex assay section, both spatial resolution and substrate zone-resolved techniques are discussed.

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